FastQCFastQC Report
Thu 2 Jun 2016
DRR028409_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028409_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568363
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47710.3042025347448263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15900.050.1252564
ATCAACG16150.048.4536133
GGTATCA13200.047.227431
CAACGCA17400.044.9741945
GTATCAA22550.040.503611
AACGCAG19100.039.4525346
GTACATG36000.038.8619461
ACGCAGA20800.035.534777
CGCAGAG20550.034.9103288
TATCAAC24100.034.5833472
TACATGG37150.033.5548932
GAGTACA27200.022.9191911
CATGGGG36450.022.660444
ACATGGG52100.021.973253
GTATTAG12250.021.3027741
GCAGAGT29800.020.9134989
GTATTAT15300.020.3726161
GTATAAC7450.019.4600281
AGTACAT28400.019.3946292
GTATATA18000.018.927581
Produced by FastQC (version 0.11.5)