FastQCFastQC Report
Thu 2 Jun 2016
DRR028409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568363
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.22781715712497683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31890.20333302940709513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.10960472798707953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.086.712721
GTATCAA50400.066.46731
ATCAACG59500.055.422143
TCAACGC59650.055.161274
CAACGCA61500.052.3468555
TATCAAC64450.051.1655122
AACGCAG64000.050.528646
ACGCAGA71100.045.38097
CGCAGAG71450.045.2600868
AACGCCG1601.0095391E-940.7854466
GCAGAGT83200.037.8224079
GTACATG36400.034.8604741
TACATGG39950.030.6590542
CGCCGAG2003.253408E-729.0029878
ACATGGG42150.028.542983
ACGCCGA2405.27034E-827.19037
GTGGTAT13000.026.2150731
TGGTATC12000.025.3662852
CAGAGTA81350.024.956479
CATGGGG37050.024.6474024
Produced by FastQC (version 0.11.5)