FastQCFastQC Report
Thu 2 Jun 2016
DRR028407_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028407_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472830
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42580.2891032909432861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22050.053.257471
TCAACGC20650.051.2288364
ATCAACG21450.049.654313
GGTATCA13250.048.6909451
CAACGCA22050.046.9968035
AACGCAG22450.045.5123066
GTACATG44050.044.1024781
TATCAAC26300.044.0879482
TACATGG42300.039.918232
ACGCAGA26300.037.474767
CGCAGAG26600.034.8725748
TACGACC1251.2706163E-534.7794654
ACATGGG44750.034.0011143
CATGGGG35500.032.656784
TAGCGCA1652.3952616E-630.7446639
CTACGCT1100.00970089526.348084
ATGGGGG30450.025.9406055
TCTACGC1400.001003330625.8766983
GCAGAGT33500.025.3102689
TAAACCG1450.001229966824.9886535
Produced by FastQC (version 0.11.5)