FastQCFastQC Report
Thu 2 Jun 2016
DRR028407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472830
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53720.3647399903586972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.29745456026832695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.1813515477006851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.089.037171
GTATCAA50350.073.170381
ATCAACG64400.056.719913
TCAACGC64850.055.879294
TATCAAC67800.053.7686542
CAACGCA67450.053.632425
AACGCAG69700.051.9011046
ACGCAGA78100.046.2260867
CGCAGAG78950.045.5447588
GCAGAGT88500.040.1385469
GTACATG39400.039.390241
TACATGG39400.036.0537722
ACATGGG38900.035.5856253
ACCGCGT850.00274330834.1153538
CATGGGG33550.030.4591264
GTGGTAT10900.029.2750231
CAGAGTA87900.024.494949
TGGTATC12200.024.35652
ATGGGGG27150.023.2304825
CAGTGCG2552.7101087E-622.7435689
Produced by FastQC (version 0.11.5)