FastQCFastQC Report
Thu 2 Jun 2016
DRR028405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634199
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17870.28177275587000294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10180.16051744010949245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.058.3214261
GTATCAA10700.052.8560941
TCAACGC9850.051.499794
GTACATG18600.050.287611
AACGCAG10350.049.0118756
ATCAACG10550.048.7696383
CAACGCA10500.048.3117075
TACATGG17350.046.795022
CGCAGAG10300.045.7355738
ACGCAGA11100.044.3980567
TATCAAC12550.043.898632
GCTACGT700.001058673841.4100343
TTAGCGC750.001485659538.6524288
CTAGTAC951.031175E-438.140823
GCAGAGT12200.033.2664349
ACATGGG21300.032.3212133
CATGGGG15150.031.0916944
GAGTACA11600.026.8778611
CCTAGTA1409.998144E-425.8894672
ATGGGGG11300.025.6522355
Produced by FastQC (version 0.11.5)