FastQCFastQC Report
Thu 2 Jun 2016
DRR028405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634199
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.5264908963905651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30390.4791871321146833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.25622872316102674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.099.304791
GTATCAA30750.070.307741
TCAACGC38100.056.686274
ATCAACG38350.056.3167423
CAACGCA39150.054.623515
TATCAAC39600.054.173032
AACGCAG39600.054.0027856
ACGCAGA43500.048.827867
CGCAGAG43950.048.3279158
GCAGAGT48750.043.1233759
GTGGTAT5100.039.830871
GTACATG15650.038.0129431
TACATGG14750.037.8342632
ACATGGG16900.034.736413
TGGTATC5850.034.6887632
CATGGGG13800.030.4603964
ATGCGGC1100.00967424826.3606836
CAGAGTA49450.024.3349889
ATAACCG1801.5205845E-424.1639615
GTGGACC3052.039269E-823.7622073
Produced by FastQC (version 0.11.5)