FastQCFastQC Report
Thu 2 Jun 2016
DRR028404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670979
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47610.28492279077115873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15700.047.9979174
GGTATCA13750.046.3900871
ATCAACG16750.044.9904483
CAACGCA17400.043.3136675
AACGCAG18600.040.1308366
GTATCAA22000.036.9012071
GTACATG36200.036.2422561
CGCAGAG21100.034.690138
ACGCAGA21350.034.6233637
TATCAAC23950.032.3786162
CGATACC9050.032.03543145
TACATGG39300.030.796682
CAACGCG950.00473800230.5133786
ACGCGGA1504.412277E-528.988588
ACATGGG44600.026.4822253
CATGGGG38600.023.4645084
GCAGAGT31850.021.845149
GAGTACA28100.019.3464011
TGAACCG2307.989323E-418.9044635
TAATACA23150.018.1537234
Produced by FastQC (version 0.11.5)