FastQCFastQC Report
Thu 2 Jun 2016
DRR028404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670979
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28770.172174515658186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.15332329131604885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.076.815341
GTATCAA40050.061.014281
TCAACGC44050.054.6213344
ATCAACG44300.053.9858973
CAACGCA45250.052.403385
AACGCAG48000.049.854326
TATCAAC51500.046.5790752
ACGCAGA53450.044.499617
CGCAGAG54050.043.73738
TAGACCG1051.8519377E-434.531135
GCAGAGT68750.033.436189
GTACATG34100.032.108991
TACATGG37400.028.8725282
ATAGTCG1050.007701932527.6249056
ACATGGG39500.025.3193233
CAGAGTA64500.023.1599489
TACGACA2104.3270746E-420.706284
CATGGGG33950.020.4928134
TGTACGA2155.0712883E-420.224742
CCTATAC6201.8189894E-1218.7024463
Produced by FastQC (version 0.11.5)