FastQCFastQC Report
Thu 2 Jun 2016
DRR028402_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028402_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214732
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6950.32365925898329084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6180.28780060726859524No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG203.860544E-4108.75932
TACGATT300.001940183272.438457
GGTATCA2900.065.0055541
ACGATTG400.006064759654.328848
GTATACG400.006064759654.328844
TACTAGG450.00966123248.2922977
GTATCAA3950.047.7255972
TCAACGC4300.047.169225
ATCAACG4250.046.0197224
GTGGTAT1401.4044417E-841.432111
AACGCAG4800.040.7466287
GTACATG7150.040.562911
CAACGCA5050.040.163896
AGCTAGT800.002032190636.2530981
TATCAAC5200.036.2192273
ACGCAGA5400.036.2192278
TACATGG7500.033.8362242
CGCAGAG5250.033.1301969
GAGTACA5550.031.354031
CATGGGG6050.031.1305714
Produced by FastQC (version 0.11.5)