FastQCFastQC Report
Thu 2 Jun 2016
DRR028402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214732
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.5979546597619358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.5909692081292028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.3376301622487566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.10897304547063315No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.10711025836857106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.0104.060261
ACCGCTC259.376275E-487.000858
GTATCAA11450.066.562881
CAAGACG502.0088896E-457.9870534
CGCGAAC400.006044208554.3755268
ATCAACG14200.053.5971873
TCAACGC14750.051.5986444
CAACGCA15250.049.4430965
CCGCGAA450.00962853848.33387
AACGCAG15650.048.179386
TATCAAC16200.047.427682
CCTAGAC951.91674E-645.779253
ACGCAGA16950.044.0564737
CGCAGAG16800.044.018288
GTACATG7200.040.3251341
GCAGAGT18800.038.56429
GTGGTAT2300.037.870561
GCCCATA800.002021362836.2926181
TACATGG8000.034.4298132
TGGTATC2353.45608E-1133.9285932
Produced by FastQC (version 0.11.5)