FastQCFastQC Report
Thu 2 Jun 2016
DRR028401_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028401_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934289
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56330.2912181168377631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14500.048.4717864
CAACGCA15450.046.43295
ATCAACG15400.045.6378333
AACGCAG17850.039.783866
GGTATCA12950.038.0621721
GTATCAA20800.036.5915151
ACGCAGA20500.034.288487
CGCAGAG20700.032.907828
TATCAAC24700.029.047832
GTACATG34500.024.1620161
TCTGTCG5500.023.7165478
GTGTACG1550.001811121423.3825951
CGATACC9000.020.934431145
GGACGGT1750.003670649420.7038466
AGTACCG2506.0014096E-520.289775
TACATGG40400.020.0914882
CTAGTAC4954.783942E-1019.0288333
ACATGGG41600.018.9848523
GTATTAG17850.018.6798761
CTGTCGC6750.018.251489
Produced by FastQC (version 0.11.5)