FastQCFastQC Report
Thu 2 Jun 2016
DRR028401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934289
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.17375893674626697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.14103373384225418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.077.3039551
GTATCAA44750.061.2496071
TCAACGC47800.056.5551454
ATCAACG48450.055.9459953
CAACGCA48850.055.510855
AACGCAG51300.053.0010836
TATCAAC55450.049.0140882
ACGCAGA59150.045.476817
CGCAGAG59250.045.155318
GCAGAGT79400.032.965349
GTACATG35450.028.0226291
TACATGG42500.023.0216272
TGTACCG1952.6146305E-422.3093785
CCGGTCG1650.002600588121.9713579
ACATGGG43500.020.8262923
CAGAGTA72400.020.2294319
GTGGTAT14100.019.5420021
AGAGTAC64150.018.56104710-14
TAGATCG2000.00791522918.1263715
TGGTATC12250.017.74912
Produced by FastQC (version 0.11.5)