FastQCFastQC Report
Thu 2 Jun 2016
DRR028400_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028400_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393372
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40230.2887240449786561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19350.052.800074
ATCAACG19500.051.2791523
CAACGCA21000.049.0000725
GGTATCA13500.047.793811
AACGCAG21350.047.5179636
GTATCAA22000.045.804441
GTACATG37000.040.5587041
TATCAAC24750.040.1191062
ACGCAGA24550.039.555997
CGCAGAG24650.037.3375438
TACATGG39050.035.0789532
ACATGGG45550.028.1566683
GCAGAGT31900.026.8100269
CGATACC10800.026.170706145
GAGTACA27700.024.8633291
CATGGGG35100.024.7725374
CTATCGT1500.001497214724.1593179
GTACCGT1650.00260868821.959066
CGTATAT2053.6687165E-421.2184091
TGTACCG1800.004319994720.1291355
Produced by FastQC (version 0.11.5)