FastQCFastQC Report
Thu 2 Jun 2016
DRR028400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393372
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.261739147908814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31550.22642912301955256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.130116006350063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.085.689731
GTATCAA43300.064.982841
ATCAACG51450.054.3740653
TCAACGC51550.054.268594
CAACGCA53850.051.2980235
TATCAAC55600.050.445922
AACGCAG55050.050.3115166
ACGCAGA62300.044.2238857
CGCAGAG63050.043.927828
GCAGAGT71750.037.995079
GTACATG36600.037.4486051
TACATGG36800.036.434582
ACATGGG38050.033.3329123
GTGGTAT9650.027.805411
CATGGGG31200.027.410524
CAGAGTA71700.024.7746939
ATATCGC2051.2902014E-524.7574256
GAGTACA27100.023.0135751
TATATCG1902.1929726E-422.8959675
TGGTATC11200.021.3543222
Produced by FastQC (version 0.11.5)