FastQCFastQC Report
Thu 2 Jun 2016
DRR028399_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028399_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775431
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51310.28900024839039085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20900.050.6221544
ATCAACG21350.050.235433
GTATCAA24800.047.351361
CAACGCA22900.046.199695
AACGCAG24150.043.509586
GGTATCA14400.041.781571
GTACATG47950.038.852021
TATCAAC29150.037.5423552
ACGCAGA28400.036.489177
CGCAGAG28000.035.457568
TACATGG49050.034.8703352
ACATGGG53800.031.2501413
CGATACC11750.030.839193145
CATGGGG43550.029.618694
GCAGAGT39750.022.7893269
GTATAAG9750.020.8172111
GTATATA16650.020.462221
GAGTACA33400.020.4009551
TATACTG14400.018.1159765
AGAGTAC32000.017.6630765
Produced by FastQC (version 0.11.5)