FastQCFastQC Report
Thu 2 Jun 2016
DRR028399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775431
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.22918378692272467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34540.1945443106490762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19240.10836805260243851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.083.005571
GTATCAA50450.060.0924221
TCAACGC58500.051.416584
ATCAACG59400.050.3935053
CAACGCA60500.049.255695
AACGCAG63000.047.3011056
TATCAAC66500.044.7951662
GTACATG43550.041.9678731
ACGCAGA71200.041.5487
CGCAGAG73700.040.4337778
TACATGG46950.035.6604922
ACATGGG48850.034.7186053
GCAGAGT84850.034.1804779
CATGGGG41450.028.851634
GTACGAC2101.55948E-524.1595973
CAGAGTA81850.023.8288789
GTGGTAT15400.023.547991
ACCGCGT1600.002174878722.6579178
TGGTATC14800.022.0374722
GAGTACA33150.021.003531
Produced by FastQC (version 0.11.5)