FastQCFastQC Report
Thu 2 Jun 2016
DRR028398_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028398_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663279
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47890.28792523683639365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20750.057.2698144
ATCAACG21750.053.970423
GTATCAA25500.050.598431
CAACGCA23500.050.2627145
AACGCAG24900.047.1471026
GGTATCA14800.043.5898671
TATCAAC30000.042.2774242
ACGCAGA27900.041.55937
CGCAGAG28450.039.739378
GTACATG45550.039.306631
TACATGG46550.036.121022
GCAGAGT36450.028.4334939
ACATGGG56500.028.2146243
CATGGGG44650.026.290184
CGATACC11350.025.538975145
GAGTACA33250.020.7104341
TATAACG1800.004318916220.1302857
AGTACAT35600.018.1188962
GTATAAG9700.017.9348071
GTATTAG13900.017.7305151
Produced by FastQC (version 0.11.5)