FastQCFastQC Report
Thu 2 Jun 2016
DRR028398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663279
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35010.21048783757866238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29830.17934453570327047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.083.8722841
GTATCAA48350.060.905091
ATCAACG56250.051.6692853
TCAACGC56950.051.0341874
CAACGCA58550.048.5532045
AACGCAG61300.046.7299546
TATCAAC62900.046.2066352
ACGCAGA69200.041.1855777
CGCAGAG70700.040.4143458
AACGCCG1553.4642653E-837.4297566
GTACATG45950.034.252971
GCAGAGT82900.034.029369
TACATGG47350.032.1447372
TCGTACG950.00473497930.517359145
ACATGGG48700.030.5095273
CATGGGG40850.029.0979464
CAGAGTA81300.022.389379
GAGTACA34550.020.993071
GTGGTAT13550.020.3408131
ATGGGGG31450.018.6776875
Produced by FastQC (version 0.11.5)