FastQCFastQC Report
Thu 2 Jun 2016
DRR028397_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028397_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678142
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49200.2931813875107112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG21900.052.2796863
TCAACGC22500.050.885564
GTATCAA24400.050.7991181
CAACGCA24900.045.400255
AACGCAG25900.043.925826
GGTATCA14000.041.937921
GTACATG46250.040.9052121
TATCAAC29450.039.623382
ACGCAGA29700.037.0868647
CGCAGAG29550.036.0500458
TACATGG48050.035.447482
CGATACC10350.033.612347145
CGACGCA1100.00969710626.3504054
GCAGAGT38250.026.3371229
CATGGGG46150.025.7508834
ACATGGG60750.025.6455613
ACCGCGT1801.5254505E-424.1559838
TATACCG1550.001813970223.3760595
GAGTACA34300.020.9214121
CAGAGTA36300.017.7692189
Produced by FastQC (version 0.11.5)