FastQCFastQC Report
Thu 2 Jun 2016
DRR028397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678142
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40360.24050408129943712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.20480984326713708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.11709378586555845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.089.880241
GTATCAA51750.063.7725681
ATCAACG63800.051.575613
TCAACGC64000.051.3011934
CAACGCA66250.048.804715
TATCAAC69950.046.9374732
AACGCAG69250.046.7951136
CGCAGAG76800.041.8172268
ACGCAGA77100.041.7485437
GCAGAGT86900.035.8724829
GTACATG45500.035.708321
TACATGG46050.033.681652
ACATGGG48800.031.189523
AACGCCG1907.1624545E-626.7089736
CATGGGG43400.026.2191814
GTGGTAT12800.025.4997181
CAGAGTA85300.024.9868229
GTCGTAC1751.2524007E-424.8682961
GTACGAC1801.5239479E-424.15953
TGGTATC13800.022.0586762
Produced by FastQC (version 0.11.5)