FastQCFastQC Report
Thu 2 Jun 2016
DRR028396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004722
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28930.28794034568766286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15250.057.972514
GTATCAA17000.057.557741
ATCAACG15950.055.87983
CAACGCA16750.053.216235
AACGCAG17600.050.234376
TATCAAC19250.048.937832
GGTATCA9950.042.9781571
ACGCAGA21050.041.663167
GTACATG31700.040.469971
TACATGG32950.037.6073532
CGCAGAG23200.035.9276438
ACATGGG38700.030.3329373
GTACCGT1204.0681454E-430.1955246
TGTACCG1753.7871068E-628.9877015
CGTTAAT1255.168165E-428.9862542
GCAGAGT28300.028.6861429
CATGGGG31800.027.57344
GAGTACA23200.024.3683151
AGTACAT24750.022.2520732
GGACCGC2256.8873167E-419.3251366
Produced by FastQC (version 0.11.5)