FastQCFastQC Report
Thu 2 Jun 2016
DRR028396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004722
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34200.34039266583194155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.24354995710256172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.1462095982769363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.078.192751
GTATCAA29250.065.2266851
TCAACGC35050.053.9707834
ATCAACG35600.053.1369633
CAACGCA36150.051.3337175
TATCAAC37100.050.9885672
AACGCAG37050.050.0867466
ACGCAGA43100.042.887827
CGCAGAG46450.039.326558
GTACATG30900.038.9713131
TACATGG34100.036.3452722
GCAGAGT50750.035.423119
ACATGGG35550.032.212453
TAGATCG1204.0617253E-430.2036785
GTGGTAT7250.029.0171951
CATGGGG31000.027.1208024
ACGCCGA1751.257046E-424.8533127
ACACGCG1500.001495619524.1629416
AACGCCG1902.195469E-422.8912076
GTATAGC2552.6932012E-622.7585851
Produced by FastQC (version 0.11.5)