FastQCFastQC Report
Thu 2 Jun 2016
DRR028395_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028395_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847740
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55630.30107049693138643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG18100.045.6362843
TCAACGC18600.044.410714
CAACGCA19700.042.6700335
GTATCAA22050.040.1051861
AACGCAG21900.037.718686
GTACATG41600.037.6365431
GGTATCA15200.037.196351
TACATGG44400.033.4608962
TATCAAC26150.033.2564742
CGCAGAG24300.031.6130358
ACGCAGA25150.030.5429467
TGTACCG1703.0247302E-629.838735
ACATGGG48100.029.2240663
CATGGGG37750.020.7301584
GTATTAG17500.020.2958551
GAGTACA33350.019.7785681
GCAGAGT37200.019.0929649
CATGGGA19600.018.8542774
CAGAGTA34250.017.3517599
GTATATA23950.017.2511731
Produced by FastQC (version 0.11.5)