FastQCFastQC Report
Thu 2 Jun 2016
DRR028395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847740
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.18281792892939483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.15803089179213525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.075.062971
GTATCAA48300.062.7614481
TCAACGC54800.054.8844344
ATCAACG55100.054.7171363
CAACGCA57150.051.8807535
TATCAAC60650.049.7100452
AACGCAG60200.049.372666
CGCAGAG67200.044.013928
ACGCAGA67500.043.8183067
GCAGAGT82350.035.3004269
GTACATG42800.030.6689261
AACGCCG1453.4941128E-529.9973146
GTGGTAT13950.026.5130541
TGGTATC13200.026.3541452
TACATGG48150.026.0394292
ACATGGG47850.025.8997633
CAGAGTA78200.023.3610829
GTCTCGC5550.022.213641
CATGGGG38550.021.0559714
ATACGGG3401.5175374E-619.189467
Produced by FastQC (version 0.11.5)