FastQCFastQC Report
Thu 2 Jun 2016
DRR028394_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028394_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495996
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42300.2827547667239752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.047.0112651
TCAACGC16050.045.600114
ATCAACG16400.044.625443
CAACGCA17150.042.6767545
GTATCAA18800.042.4059941
AACGCAG18550.039.454546
TATCAAC21800.034.5697632
GTACATG35550.034.453991
CGCAGAG21250.032.7397658
TACATGG38450.031.8500352
ACGCAGA22700.031.285927
ACATGGG39950.030.6531353
CGTACAC2101.5622109E-524.1537043
CATGGGG34900.024.0853044
GAGTACA26900.021.8235381
GCAGAGT28350.021.2186379
GTGTAAG10100.020.8098551
AGTACAT29850.019.4207152
GTCTTAC7200.018.1189251
CTGTCGC5852.3646862E-1017.3446129
Produced by FastQC (version 0.11.5)