FastQCFastQC Report
Thu 2 Jun 2016
DRR028394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495996
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.17312880515723306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.14752713242548776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.072.705371
GTATCAA37000.060.7702751
ATCAACG41300.053.8729863
TCAACGC41150.053.717124
CAACGCA42650.051.0021135
AACGCAG45500.048.4449126
TATCAAC47600.047.1995052
ACGCAGA50650.043.375977
CGCAGAG50500.043.3612258
GTACATG35050.035.386641
GCAGAGT61750.035.1091429
TACATGG38050.032.3800132
ACATGGG38900.029.4367713
TATACCG1358.0793805E-426.8548185
CATGGGG31350.025.1983264
AACGCCG1450.00122593825.0027626
CAGAGTA59600.023.6015999
GAGTACA26050.022.8316521
GTGGTAT11100.021.5636461
TAGACAG11200.021.3639685
Produced by FastQC (version 0.11.5)