FastQCFastQC Report
Thu 2 Jun 2016
DRR028393_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028393_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180719
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5900.326473696733603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.1992042895323679No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG3160.17485709858952297No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC2930.16213015786939947No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT2700.14940321714927593No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC2680.1482965266518739No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT2550.14110303841876062No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2260.125056026206431No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT2080.11509581172981258No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2040.11288243073500849No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC1980.10956235924280235No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1970.10900901399410133No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT1850.10236887100968908No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT100.0070927283144.954387
GCTCTAG550.0118.599041
GGTATCA1400.098.361911
TCATGGA300.001935565872.477192
ATACCTT406.6587134E-572.477196
AACCTAT350.003566189762.1233066
ACACAGA651.0920532E-555.7516828
CATGGGA3150.055.2207154
GTACATG5850.054.512761
GAATACT400.006050373454.357898
GCCGCGT400.006050373454.35789145
TACATGG5450.053.194272
ATCAACG2950.051.593933
TCAACGC3100.049.0974544
GTATCAA3450.048.318131
GAATCAA604.941853E-448.3181272
CAACGCA3500.043.4863175
ACATGGG6300.042.5659683
AAATCCG700.001055960941.415545
TGGGAAT700.001055960941.415546
Produced by FastQC (version 0.11.5)