FastQCFastQC Report
Thu 2 Jun 2016
DRR028393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180719
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.5112910097997444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.47753694962898197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.2882928745732325No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT3680.20363105152197608No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC3420.18924407505574953No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC3300.18260393207133727No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG3120.1726437175947189No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT2900.1604701221232964No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT2800.15493666963628616No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2780.15382997913888413No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA2610.14442310991096677No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC2310.1278227524499361No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC2300.12726940720123509No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2280.12616271670383303No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT2240.12394933570902894No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT2230.12339599046032793No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA2160.11952257371942077No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC2130.1178625379733177No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT1990.11011570449150339No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1960.10845566874540032No Hit
CAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACC1870.10347556150709111No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG1860.1029222162583901No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1029222162583901No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGC100.007064467145.148248
AATTCGT100.007064467145.148246
GGTATCA3350.0116.985151
GTGGTAT607.2759576E-1296.765491
TGGTATC607.2759576E-1296.6316152
GCTCTAG406.0600723E-790.717651
TGGGTGC300.00192534372.574126
GGGAACC300.00192534372.574127
TGGGAAC350.003547381162.206396
GTATCAA8000.056.2449381
GTATAAA400.00601850654.430591
ACCCGGT400.00601850654.430591
CATGGGA3200.052.090484
CATGGGT858.9060813E-751.1579134
ATCAACG8650.051.108633
TCAACGC8750.050.5245324
CAACGCA8950.049.4639245
TACATGG5950.048.7218252
ATGATGT450.00958765748.3827449
ATAGATG901.3082536E-648.3827446
Produced by FastQC (version 0.11.5)