FastQCFastQC Report
Thu 2 Jun 2016
DRR028392_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028392_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637930
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47600.2906107098593957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19400.052.2850954
ATCAACG19400.052.283493
CAACGCA21450.047.9695785
GTATCAA23000.047.8961371
GGTATCA12800.044.7303051
AACGCAG23050.043.6980446
GTACATG43100.041.534061
TATCAAC27950.038.6298142
TACATGG44800.038.1726342
ACGCAGA26700.036.642127
CGCAGAG27150.034.7012338
CATGGGG41050.027.3571034
ACATGGG55600.026.191453
GCAGAGT36100.024.0934359
GTCTAGT8450.022.299821
GAGTACA31650.021.29581
ATGGGGG31300.018.7519115
GTATTAG13750.017.9209461
CTATACT12050.017.436634
TAGTACG3804.413543E-617.159734
Produced by FastQC (version 0.11.5)