FastQCFastQC Report
Thu 2 Jun 2016
DRR028392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637930
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.19762749323841677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.1689937909434469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.075.9712751
GTATCAA49750.063.5644651
ATCAACG57800.054.4190943
TCAACGC58300.053.7037474
CAACGCA60250.051.3892675
AACGCAG62700.049.496886
TATCAAC63450.049.4590382
ACGCAGA70550.043.5783127
CGCAGAG70950.043.1282278
GCAGAGT84400.035.3102659
GTACATG42250.030.7289181
GTGGTAT12950.028.5641561
TACATGG45100.027.8009072
ACATGGG46750.026.6646733
TGGTATC13400.024.8795472
CAGAGTA83400.024.8657519
AACGCCG1500.001493405324.1702046
CATGGGG40700.022.9712524
GTATAAC8150.020.4687461
AGAGTAC77600.018.74973110-14
Produced by FastQC (version 0.11.5)