FastQCFastQC Report
Thu 2 Jun 2016
DRR028391_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028391_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039512
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60950.29884599845453225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18900.054.056874
ATCAACG19350.052.7997323
CAACGCA20550.049.7214285
AACGCAG22300.045.493446
GTATCAA27100.043.866041
ACGCAGA23350.041.5866787
GGTATCA15700.038.7822951
CGCAGAG24050.037.9657338
TATCAAC30350.034.8636782
GTACATG37850.033.1309661
TACATGG42150.027.3387472
GCAGAGT35050.023.9849249
TCTGTCG6150.023.5662888
ACATGGG46450.023.087113
CATGGGG38450.020.729554
CTGTCGC6750.020.3994379
CCGTGCG1900.00590323719.0714959
GTACTAG6251.8189894E-1218.5564041
GTCTCGC5251.0440999E-917.9489041
ATATACG3252.2154854E-517.8361113
Produced by FastQC (version 0.11.5)