FastQCFastQC Report
Thu 2 Jun 2016
DRR028391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039512
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.16945230035420236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.1422399083702376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.073.362671
GTATCAA52850.064.237271
TCAACGC58050.058.1802754
ATCAACG58050.058.0554283
CAACGCA60200.055.7617385
AACGCAG61800.054.2007526
TATCAAC66450.050.4984552
CGCAGAG69900.047.5050858
ACGCAGA70550.047.1701747
GCAGAGT84700.038.6907239
GTACATG40250.032.080441
TACATGG43200.028.5205082
ACATGGG43850.026.1143723
TGGTATC15150.023.9193552
CAGAGTA81600.022.9235239
CATGGGG37800.022.6246724
GTGGTAT18500.022.3506071
GTCTCGC5400.021.4937611
GTATATA22100.020.6792351
TAGACCG1850.00504771319.5952195
Produced by FastQC (version 0.11.5)