FastQCFastQC Report
Thu 2 Jun 2016
DRR028390_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028390_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695530
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50720.2991395021025874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG21450.049.3179633
TCAACGC21800.048.5261574
GTATCAA25050.044.8402331
GGTATCA16450.043.6127171
CAACGCA24050.043.385025
GTACATG50200.041.8637541
AACGCAG25400.041.0791246
TACATGG50950.038.8283962
ACGCAGA27650.037.47657
TATCAAC29950.035.8093032
CGCAGAG29200.034.990848
ACATGGG60050.032.4577833
CATGGGG45900.030.6244724
GCAGAGT36150.025.8605739
GTACGAC1650.00261059921.9566273
CGTGTCG2003.1111643E-421.742855145
GAGTACA38500.021.6461661
TAGTGCG2353.723094E-521.5867339
AGTACAT38550.020.8655382
CCGTATA1850.005062221519.5852572
Produced by FastQC (version 0.11.5)