FastQCFastQC Report
Thu 2 Jun 2016
DRR028390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695530
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41160.2427559524160587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35340.2084304022930883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.11825210995971762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.084.204671
GTATCAA56650.063.1143071
ATCAACG64400.055.031223
TCAACGC65500.054.107034
CAACGCA67300.051.815185
AACGCAG70100.050.1592186
TATCAAC72250.048.852872
ACGCAGA77450.045.3055087
CGCAGAG79050.044.1133738
GCAGAGT92000.037.037119
GTACATG51700.035.0695571
TACATGG51150.034.5734832
ACATGGG55450.031.368653
CATGGGG44650.027.5939354
CAGAGTA91000.024.5378429
AACGCCG2401.5995283E-624.1660566
ACGCCGA2603.2070457E-622.3071297
GTGGTAT13350.021.729991
ATGCGAC2155.058991E-420.2320489
GAGTACA39600.019.779231
Produced by FastQC (version 0.11.5)