FastQCFastQC Report
Thu 2 Jun 2016
DRR028389_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028389_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898151
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55380.29175761043246823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17650.053.776774
ATCAACG18600.050.2523573
CAACGCA19250.048.5542345
GTATCAA23150.043.8300171
AACGCAG21650.043.171786
GTACATG39550.037.016851
TATCAAC26600.036.7771222
CGCAGAG24100.036.6842738
GGTATCA14750.035.8695071
ACGCAGA25800.035.1069377
TACATGG44250.031.769772
ACATGGG56300.022.7795183
CATGGGG39850.021.8183084
GCAGAGT36450.021.2744269
GTATAAG12900.019.6640471
GTATTAG15850.019.6622751
CTAGTAC5851.2732926E-1118.5786883
AGAGTAC27750.018.015835
ATGGGGG27100.016.8437755
TATTAGG16450.016.7395322
Produced by FastQC (version 0.11.5)