FastQCFastQC Report
Thu 2 Jun 2016
DRR028389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898151
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35030.1845480154107866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30510.1607353682610077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.073.639551
GTATCAA51200.063.741621
TCAACGC55950.057.903114
ATCAACG57250.056.4616853
CAACGCA58350.054.9207045
AACGCAG61800.051.3854646
TATCAAC64700.049.848282
ACGCAGA69100.045.747057
CGCAGAG68900.045.6693848
GCAGAGT84800.036.1658949
GTACATG43000.031.708081
AACGCCG1453.491115E-530.001156
TACATGG48150.027.8464552
ACATGGG47450.027.0353183
CATGGGG38600.023.0946964
CAGAGTA82250.022.4780679
CCGTTCA2053.6671656E-421.2203259
CGTGTCG2857.1011236E-620.3516589
TGGTATC14600.019.3600372
AGAGTAC76350.018.25908710-14
Produced by FastQC (version 0.11.5)