FastQCFastQC Report
Thu 2 Jun 2016
DRR028388_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028388_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657745
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48410.2920232001906204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22050.052.582814
GTATCAA23800.052.0798721
ATCAACG22550.051.095543
GGTATCA16000.047.5685651
CAACGCA24550.046.9329725
AACGCAG25450.045.273266
GTACATG50150.041.193121
TACATGG50400.039.11322
TATCAAC30700.038.7162
ACGCAGA30750.037.7068677
ACATGGG53600.035.2864653
CGCAGAG32650.034.8477868
CATGGGG44250.032.916624
GCAGAGT40450.026.6947889
GAGTACA37000.023.7046971
ATGGGGG33900.022.4451265
AGTACAT38050.021.3328232
GTATAAG9700.020.9236321
TACGGAT2857.129831E-620.3418627
CTATACT10800.018.1164234
Produced by FastQC (version 0.11.5)