FastQCFastQC Report
Thu 2 Jun 2016
DRR028388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657745
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39250.2367674159777288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.1914649116721812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.1138293283948979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.078.17081
GTATCAA47050.064.5880661
TCAACGC54350.054.6723484
ATCAACG54800.054.3556483
CAACGCA55050.052.81145
AACGCAG57600.050.3475276
TATCAAC61400.048.6308942
ACGCAGA66300.043.1940777
CGCAGAG67350.042.735978
GTACATG49050.039.4793661
TACATGG49700.038.3515362
GCAGAGT77650.036.3202449
ACATGGG55250.033.3184553
CATGGGG44750.031.0950744
ACGCCGA2207.490562E-726.3637967
GAGTACA36900.023.3893681
AACGCCG2502.2806016E-623.200146
CAGAGTA75550.022.1675729
TATAACG2003.1117068E-421.742264
AGTACAT40050.020.991282
Produced by FastQC (version 0.11.5)