FastQCFastQC Report
Thu 2 Jun 2016
DRR028387_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028387_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877933
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55350.2947389496856384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21150.051.7308854
ATCAACG21850.050.406563
CAACGCA23400.047.9979135
GTATCAA27000.044.8306851
AACGCAG25300.044.6821256
GGTATCA17000.041.356661
TATCAAC28800.040.260622
GTACATG45750.038.3395421
ACGCAGA28850.037.9290967
CGCAGAG28450.035.9161538
TACATGG46300.034.278052
CATGGGG47100.026.1523864
ACATGGG60300.025.5951233
GCAGAGT39500.023.4842919
GAGTACA34050.020.6479641
TCTGTCG5950.019.4874948
GTATAGG8250.019.32821
GTATAGA15650.018.525431
ATGGGGG38000.018.1153435
GTACGGA2450.001221383617.7465926
Produced by FastQC (version 0.11.5)