FastQCFastQC Report
Thu 2 Jun 2016
DRR028387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877933
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.1928183806344529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.1717846163840776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.087.5659941
GTATCAA54050.059.974451
TCAACGC61850.051.674434
ATCAACG62050.051.624673
CAACGCA63900.049.3584865
AACGCAG66300.047.8998376
TATCAAC69850.045.75612
ACGCAGA74150.042.633287
CGCAGAG75950.041.7183468
GCAGAGT88600.035.4346059
GTACATG44000.034.7763631
TACATGG46750.032.2447172
ACATGGG48650.030.3895423
GTACCGT1358.0815173E-426.8540426
CATGGGG43750.025.8418374
CAGAGTA85950.024.548259
GAGTACA34400.021.2920441
ATGGGGG30900.019.9449945
TGTACCG1900.005886996619.0805035
AGAGTAC82150.019.05502910-14
Produced by FastQC (version 0.11.5)