FastQCFastQC Report
Thu 2 Jun 2016
DRR028386_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028386_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895386
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56370.2974064385829588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19250.053.8229144
CAACGCA20700.050.0527155
ATCAACG21100.049.1038443
AACGCAG22300.046.1365786
GTATCAA27150.045.1169551
GGTATCA16200.039.372281
CGCAGAG24750.038.9379278
ACGCAGA26150.038.5148357
GTACATG45750.037.7058561
TATCAAC30050.036.8997882
TACATGG48150.032.962852
ACATGGG55100.025.1156063
GCGTATA1500.001497071524.1602651
GCAGAGT38550.023.6858279
CATGGGG43800.022.3316884
GAGTACA33050.020.8341161
GTATAGA15450.020.6417791
GTCGTAA2205.916577E-419.7674881
CTATACT15350.017.9364764
TAAGCGT3052.7567413E-416.6287694
Produced by FastQC (version 0.11.5)