FastQCFastQC Report
Thu 2 Jun 2016
DRR028386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895386
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.19842923816045913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32170.16972796042600294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28150.074.447091
GTATCAA52050.066.872691
TCAACGC62800.054.81784
ATCAACG63350.054.3418733
CAACGCA64750.053.189365
AACGCAG66250.052.094526
TATCAAC68950.049.5078742
CGCAGAG72900.046.845128
ACGCAGA73450.046.6917657
GCAGAGT89800.037.544619
GTACATG43650.033.557951
ACGCCGT1153.1617522E-431.524136
TACATGG46450.031.0495262
ACATGGG45500.029.4678153
GTACCGT1658.4181294E-526.3656376
CATGGGG40150.026.1739984
GTACGAC1958.783471E-626.016633
CAGAGTA88200.025.5669
GTACCGG1801.5202032E-424.16856
TGTACCG2401.598286E-624.16855
Produced by FastQC (version 0.11.5)