FastQCFastQC Report
Thu 2 Jun 2016
DRR028385_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028385_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961171
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58800.29982087232576865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG23550.052.9167183
TCAACGC23300.052.861234
CAACGCA24800.049.666525
GGTATCA17450.048.180761
GTATCAA27900.047.2800831
AACGCAG30250.040.9568066
TATCAAC33350.039.1119842
GTACATG50200.038.5494841
TACATGG51350.035.9859472
ACGCAGA34050.034.6872867
CGCAGAG32900.034.579188
ACATGGG57750.030.8630243
GCAGAGT38750.026.9306139
CATGGGG44000.025.8518244
GAGTACA36950.022.7538341
TATACTG16250.019.61855
AGTACAT40850.019.3360442
GTATTAG13700.018.5164831
GTATATA20950.017.9899671
CCTATAC8450.017.1485983
Produced by FastQC (version 0.11.5)