FastQCFastQC Report
Thu 2 Jun 2016
DRR028385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961171
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.19478158712320343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34280.1747935289681522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.082.2683261
GTATCAA54600.063.484841
ATCAACG63800.053.957653
TCAACGC63700.053.8148044
CAACGCA64700.052.329635
AACGCAG70400.048.1956986
TATCAAC72400.047.4482082
CGCAGAG78150.043.2306758
ACGCAGA78750.042.90137
GTACATG49300.037.067081
GCAGAGT91500.036.130919
AACGCCG1051.8544369E-434.523586
TACATGG51850.034.2450182
ACATGGG53800.032.3302463
CATGGGG41500.026.0206554
GTGGTAT15150.024.411381
CAGAGTA91200.023.769039
TGGTATC15550.020.5070112
CCTATAC8200.020.3279723
GAGTACA37500.019.1442681
Produced by FastQC (version 0.11.5)