FastQCFastQC Report
Thu 2 Jun 2016
DRR028384_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028384_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836380
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54900.29895773205981335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21200.048.5332764
ATCAACG21350.048.5290223
CAACGCA22000.046.4428835
GTATCAA26600.042.5070231
AACGCAG23900.042.446416
GGTATCA16500.039.9737781
TATCAAC29650.035.6860432
CGCAGAG28050.035.137948
ACGCAGA28450.034.643917
GTACATG46100.034.2746731
TACATGG48050.030.4668962
TTAGCGC1358.10355E-426.8414828
ACATGGG52300.026.5988523
GACGACC14100.024.673342145
CATGGGG43200.024.152714
GCAGAGT37500.023.3868569
GAGTACA33700.021.5073991
TATACTG16950.019.6657645
GTATAAG11650.018.0422131
TTATACT21500.017.8617734
Produced by FastQC (version 0.11.5)