FastQCFastQC Report
Thu 2 Jun 2016
DRR028384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836380
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.19614676700900688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28990.15786492991646608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.078.086921
GTATCAA48200.059.438631
TCAACGC52950.053.9298974
ATCAACG53600.053.4111143
CAACGCA54350.052.420215
AACGCAG57100.050.0225566
TATCAAC60150.047.3539542
ACGCAGA66250.043.113787
CGCAGAG68450.041.7280968
GCAGAGT79100.035.4682929
GTACATG41050.031.6270451
TACATGG43750.028.1624372
ACATGGG45250.027.3890443
CATGGGG39500.024.9540584
CAGAGTA76000.024.037699
GAGTACA31200.019.9922921
AGAGTAC72350.018.06165710-14
ATGGGGG30050.017.6110065
GTATATG13500.017.1923391
GTGGTAT15500.016.8457181
Produced by FastQC (version 0.11.5)