FastQCFastQC Report
Thu 2 Jun 2016
DRR028383_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028383_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762240
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50530.2867373343017977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20800.048.7742774
GGTATCA15750.047.401731
ATCAACG21900.046.3231123
CAACGCA22700.044.055915
GTATCAA26300.042.442651
AACGCAG24300.040.558666
GTACATG45850.037.941061
TATCAAC28750.037.810872
ACGCAGA28050.034.8790137
TACATGG47600.034.1039242
CGCAGAG28650.032.630858
ACATGGG51750.030.3852753
GACGACC14500.028.48935145
CATGGGG41450.026.7481084
CCGGTCG3551.5643309E-1024.4999779
GCAGAGT37500.023.3865919
GAGTACA34600.020.5299321
GTATATA19500.020.4439871
TAGTACG3208.517982E-720.3806824
GTATTAG13800.018.9086691
Produced by FastQC (version 0.11.5)