FastQCFastQC Report
Thu 2 Jun 2016
DRR028383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762240
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44980.2552433266751407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36400.20655529326311967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.11672643907753769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.083.965041
GTATCAA50050.064.050651
ATCAACG59100.054.326713
TCAACGC59250.054.31154
CAACGCA60750.052.145715
AACGCAG64450.049.4895176
TATCAAC66550.048.0272452
ACGCAGA72750.043.2454227
CGCAGAG73200.042.8805438
GTACATG42700.037.0283161
GCAGAGT84100.036.805729
TACATGG44050.034.0581932
ACATGGG45250.033.3151633
GTACGAC1555.514941E-528.0554163
CATGGGG39700.027.9317464
AACGCCG2001.0671092E-525.3718136
CAGAGTA83450.024.236029
TATACAG15700.020.3159185
GAGTACA32100.020.1089921
CTAGCCG2359.2116406E-418.5083129
Produced by FastQC (version 0.11.5)