FastQCFastQC Report
Thu 2 Jun 2016
DRR028381_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028381_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640808
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47650.29040570255630155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG18600.055.3134163
TCAACGC18650.055.1651234
CAACGCA21000.049.3414235
AACGCAG21400.048.0820276
GTATCAA23250.045.2021981
TATCAAC25100.043.0177152
GTACATG43950.041.723061
GGTATCA13700.041.2656361
ACGCAGA24250.040.93967
CGCAGAG24250.040.0431148
TACATGG44600.039.157682
GACGACC14650.032.646835145
ACATGGG55900.029.8106253
CATGGGG44400.028.7200244
GCAGAGT33900.025.8670589
TAGATCG2001.0706457E-525.3608025
GAGTACA29900.025.210221
ATGGGGG33600.023.2905335
AGTACAT35950.020.1574652
GTATAGA11400.017.8019561
Produced by FastQC (version 0.11.5)