FastQCFastQC Report
Thu 2 Jun 2016
DRR028381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640808
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.18186161939727255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.14913384137571245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.083.530521
GTATCAA45650.063.0710641
TCAACGC54050.053.5015534
ATCAACG54000.053.282673
CAACGCA55700.051.286415
AACGCAG57450.049.8503656
TATCAAC59750.048.033752
ACGCAGA65300.043.635577
CGCAGAG66700.042.3935788
GCAGAGT79800.034.525669
GTACATG38850.031.9216651
TACATGG41500.028.291512
GTCGTAC1050.007697534727.6281091
ACATGGG44900.025.826343
CAGAGTA76350.024.5003329
CATGGGG40550.023.5924074
GAGTACA31850.020.9487861
GTGGTAT13850.020.9454981
TGGTATC12350.020.539522
CGTACAC1900.00590112919.072413
Produced by FastQC (version 0.11.5)